Two subsets of primers were successfully used to amplify two portions of the N315 chromosome. A set of primers was generated from the genome sequence of S.aureus N315, employed here as a reference strain. GenoFrag was developed for the analysis of Staphylococcus aureus genome plasticity by whole genome amplification in ~10 kb‐long fragments. Posted on 4 4 Categories PCR / Primer Design Tags BatchPrimer3, High Throughput, PCR, Primer Design, Sequencing, Web Application RJPrimers 1.0 – Transposable Element Junction based PCR Primer Design ![]() You FM, Huo N, Gu YQ, Luo MC, Ma Y, Hane D, Lazo GR, Dvorak J, Anderson OD.īMC Bioinformatics. A batch input of large number of sequences and a tab-delimited result output greatly facilitates rapid primer design and ordering process.ĭepartment of Plant Sciences, University of CaliforniaīatchPrimer3: a high throughput web application for PCR and sequencing primer design. ![]() BatchPrimer3 allows users to design several types of primers including generic primers, hybridization oligos, SSR primers together with SSR detection, and SNP genotyping primers (including single-base extension primers, allele-specific primers, and tetra-primers for tetra-primer ARMS PCR), as well as DNA sequencing primers. ![]() BatchPrimer3 is a comprehensive web primer design program using Primer3 core program as a major primer design engine to design different types of PCR primers and sequencing primers in a high-through manner.
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